Liver Enzyme

Liver Enzyme Lab

Purpose:

The purpose of this experiment is to get a line the optimal temperature of Catalase.

Procedure:

Place a small core of blended liver a baggie
Heat irrigate on a hotplate (or use ice if needed) to bring your water bath to your assigned temperature (measure with thermometer)
Place baggie with liver into water, brining both to coveted temperature.
When ready, add 2 filter musical composition discs to the liver in the baggie and let it sit in the water for 3-4 minutes.
Meanwhile, set up your apparatus, as follows, to measure O2 production (volume in mL)
*Perform dickens trials
When ready, mix the peroxide with the discs, measure the volume of the splatter produced.

Materials:

-Thermometer
-Filter paper discs
-Inverted graduated cylinder
-Hydrogen peroxide
-Cafeteria tray
-Rubber Stopper with tube
-Gas detain bottle
-Blender
-Liver
-Ziploc bag
-Hotplate
-Beakers

Temperature
(°C)Trial 1
(mL)Trial 2
(mL)Average
(mL)
0120 140130
10118115116.5
20118123120.5
30110115112.5
40171159165
501029297
60010050
7020010
80000
900.5126.



25
100000



Analysis:
looking at the graph above, we notice overall the graph increases to a peak, and past begins to decrease all the way down to zero. At 0 °C the gas production is at roughly 130 mL, however at 10°C, 20°C and 30°C the gas production remains constant- ranging from 116.5mL to 120.5mL and then finally to 112.5mL. At 40°C, there is a fulminant boom in oxygen gas production, shooting up to 165mL. After this boom, the amount of oxygen produced decreases at 50°C down to about 97mL. It continues to decrease at 60°C and 70°C going down to 50mL and then 10mL. lastly at 80°C, there is no oxygen gas produced but at 90°C we notice almost gas production, 6.25mL. The last temperature at 100°C decreases backIf you exigency to get a full essay, order it on our website: Ordercustompaper.com



If you want to get a full essay, wisit our page: write my paper