Unknown Lab Report

Unknown Lab radical: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 due Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae thoughtless from the moderaten #103 was a grand-negative Rod. The purpose of this laboratory was to fragment and severalise the genus and species of an unknown bacterium. It is of import to post an unknown micro- existence because experience how the bacteria work and how it is merged means knowing how it provoke affect humans. Unknown bacteria may similarly be employ clinically many another(prenominal) pharmaceutical drugs argon establish on products made by organisms (Katzung, B.G.2004). In ready to identify the unknown organism a series of tests were per organise. The tests utilize were glucose, sucrose, lactose fermentation; Urease, motility, MR-VP, citrate, oxidase, and indole these tests were used to identify the genus of the unknown and a McConkey nutrient agar-agar scale leaf was used to differentiate and confirm the species of the unknown. This roam will explain and give more in perspicaciousness reasons why certain tests were through and the results yielded from them. Materials & Methods On the first daylight after receiving the unknown micro-organism a streak dwelling was aseptically done as shown in exercise 2 of the laddie manual (Kleyn 9).

The streak plate proficiency is done to absorb a colony formed by a principal cell from a variety show containing millions of cells. The streak plate proficiency was also used to obligate pure cultures of the bacteria victimization a trypticase soy agar (TSA) plate and was incubated at 37 ? for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the show of the cells; if an organism appears purple below a microscope then it is say to be Gram positive, and if it appears pink then it is said to be Gram negative. To prevent the organism from pull outting contaminated,...If you want to get a full essay, enact of battle it on our website: Ordercustompaper.com

If you want to get a full essay, wisit our page: write my paper